Specifications
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Instrument Configurations |
P/N 6200: 2-Plex (Green, Yellow) P/N 62H0: 2-Plex (Green, Yellow) + HRM (High Resolution Melt) P/N 6500: 5-Plex (Green, Yellow, Orange, Red, Crimson) P/N 65H0: 5-Plex (Green, Yellow, Orange, Red, Crimson) + HRM (High Resolution Melt) P/N 6600: 6-Plex (Blue, Green, Yellow, Orange, Red, Crimson) |
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Rotor Configurations |
30 µL x 100-wells: RotorDisc™ 100 (heat-sealed plate) AVAILABLE SOON 0.1 mL x 72-wells: RotorDisc™ 72 (heat-sealed plate) 0.1 mL x 72-wells: 0.1 mL strip tubes (strips of 4, matching caps) 0.2 mL x 36-wells: 0.2 mL tube (attached cap, domed or flat) |
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Channel Information |
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Channel Blue Green Yellow Orange Red Crimson HRM |
Excite 365±20 470±10 530±5 585±5 625±10 680±5 460±15 |
Detect 460±15 510±5 555±5 610±5 660±10 >712 (LP) 510±5 |
Some Example Fluorophores Detected Biosearch Blue™, Marina Blue®, Bothell Blue®, Alexa Fluor® 350 FAM™, SYBR® Green 1, Fluorescein, EvaGreen™, Alexa Fluor® 488 JOE™, VIC™, HEX™, TET™, CAL Fluor® Gold 540, Yakima Yellow® ROX™, CAL Fluor® Red 610, Cy3.5™, Texas Red®, Alexa Fluor®568 Cy5™, Quasar™ 670, LightCycler Red640®, Alexa Fluor 633 Quasar™ 705, LightCycler Red705®, Alexa Fluor® 680 SYTO®9, LC Green®, LC Green® Plus+, EvaGreen™... | |
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Thermal Performance |
Uniformity: ± 0.01°C Resolution: 0.02°C (smallest programmable and reported thermal increment) Range: ambient to 99°C Peak Ramp Rate (air): >15°C/sec (heating), >20°C/sec cooling Accuracy: ± 0.25°C, measured 30 seconds after clock start |
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Optical System |
Fixed path length Separate color high-intensity LED (light-emitting diode) excitation source per channel Separate emission filter per channel |
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Detector |
Photomultiplier (PMT) detector with variable or automatic gain setting (sensitivity control), user selected |
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Dimensions |
370mm (14.6") wide, 420mm (16.5") deep, 560mm (22") deep door open, 275mm (10.8") high |
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Weight |
14 kg (31 lbs) |
| Electrical |
100-120VAC @ 60Hz, 200-240VAC @ 50Hz Power consumption: 8VA (idle/standby), 560VA (peak) upgrades |
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Minimum Computer |
Windows XP® OS: WinXP/Pro, Pentium®IV 2.6 GHz (desktop) or Pentium®M 1.6 GHz (laptop), 512Mb RAM. Windows Vista® OS: Vista Business edition, Intel® Core™2 Duo processor, 1 Gb RAM, DirectX 9 capable. Common: DVD/CD-ROM drive, 80 Gb HD, RS-232 serial port (desktop) or USB port with USB-RS232-serial converter (desktop or laptop), 1024 x 768 monitor (minimum), 1280 x 1024 (preferred), speakers recommended. |
| Software |
Extensive analysis, graphing and statistical functions built-in. Unlimited use software licence included, free upgrades (by web download), Windows XP, Pentium IV (2GHz) or higher PC, USB or Serial Port required |
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40 Cycle Run Time |
Typically 45 minutes to 1.5 hours (rotor and protocol dependant) |
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Supported Volumes |
5 µL to 100 µL (rotor and protocol dependant) Typical reaction volume 20 µL |
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Instrument Color |
Outback Red or Bondi Blue |
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HRM (High Resolution Melt) |
New HRM capability is optional and comprises a dedicated high-performance optical subsystem, additional hardware and specific HRM analysis software (fully integrated). Thermal uniformity ±0.01°C, Resolution ±0.02°C, Range ambient–99°C. HRM data acquisition (read) rate of 20 reads for each 0.02°C increment (=1000 reads/°C)
NOTE: Conventional DNA Melt/Anneal capability and analysis software (including auto-call genotyping capability) is standard on all models. |
| DNA Concentration Measurement |
For direct measurement of nucleic acid concentration using fluorescent dyes (e.g. PicoGreen®, RiboGreen®, etc). Comprises dedicated analysis software (standard on all models) |
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Rotor-Gene, RotorDisc, HRM and OTV are trademarks of Corbett Life Science and it’s subsidiaries. VIC is a registered trademark and FAM, JOE, ROX and TET are trademarks of Applera Corporation. SYBR, Marina Blue, Alexa Fluor, Texas Red, SYTO, Quant-iT, PicoGreen and RiboGreen are registered trademarks of Invitrogen Corporation, Carlsbad CA. BiosearchBlue is a trademark and CAL Fluor and Quasar are registered trademarks of Biosearch Technologies, Inc. Cy is a registered trademark of GE Healthcare Group Companies. Bothell Blue, Redmond Red and Yakima Yellow are registered trademarks of Epoch Biosciences, Inc. LightCycler Red670, LightCycler Red705 are registered trademarks of Roche Molecular Systems, Inc. LCGreen is a trademark of Idaho Technology Inc. EvaGreen is a trademark of Biotium, Inc. Windows is a registered trademark of Microsoft Corporation. Pentium is a registered trademark of Intel Corporation.
For research use only. Specifications subject to change without notice. ©2007 Corbett Life Science. v2.1 |