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Specifications

Instrument Configurations

P/N 6200: 2-Plex (Green, Yellow)
P/N 62H0: 2-Plex (Green, Yellow) + HRM (High Resolution Melt)
P/N 6500: 5-Plex (Green, Yellow, Orange, Red, Crimson)
P/N 65H0: 5-Plex (Green, Yellow, Orange, Red, Crimson) + HRM (High Resolution Melt)
P/N 6600: 6-Plex (Blue, Green, Yellow, Orange, Red, Crimson)

Rotor Configurations

30 µL x 100-wells: RotorDisc™ 100 (heat-sealed plate) AVAILABLE SOON
0.1 mL x 72-wells: RotorDisc™ 72 (heat-sealed plate)
0.1 mL x 72-wells: 0.1 mL strip tubes (strips of 4, matching caps)
0.2 mL x 36-wells: 0.2 mL tube (attached cap, domed or flat)
 

Channel Information

Channel 
Blue
Green
Yellow
Orange
Red
Crimson
HRM

Excite
365±20      
470±10
530±5
585±5
625±10
680±5 
460±15     

Detect
460±15      
510±5 
555±5
610±5
660±10
>712 (LP) 
510±5     

Some Example Fluorophores Detected
Biosearch Blue™, Marina Blue®, Bothell Blue®, Alexa Fluor® 350
FAM™, SYBR® Green 1, Fluorescein, EvaGreen™, Alexa Fluor® 488
JOE™, VIC™, HEX™, TET™, CAL Fluor® Gold 540, Yakima Yellow®
ROX™, CAL Fluor® Red 610, Cy3.5™, Texas Red®, Alexa Fluor®568
Cy5™, Quasar™ 670, LightCycler Red640®, Alexa Fluor 633
Quasar™ 705, LightCycler Red705®, Alexa Fluor® 680
SYTO®9, LC Green®, LC Green® Plus+, EvaGreen™...

Thermal Performance

Uniformity: ± 0.01°C
Resolution: 0.02°C (smallest programmable and reported thermal increment) 
Range: ambient to 99°C
Peak Ramp Rate (air): >15°C/sec (heating), >20°C/sec cooling
Accuracy: ± 0.25°C, measured 30 seconds after clock start

Optical System

Fixed path length
Separate color high-intensity LED (light-emitting diode) excitation source per channel
Separate emission filter per channel

Detector

Photomultiplier (PMT) detector with variable or automatic gain setting (sensitivity control), user selected

Dimensions

370mm (14.6") wide, 420mm (16.5") deep, 560mm (22") deep door open, 275mm (10.8") high

Weight

14 kg (31 lbs)

Electrical

100-120VAC @ 60Hz, 200-240VAC @ 50Hz
Power consumption: 8VA (idle/standby), 560VA (peak) upgrades

Minimum Computer 

Windows XP® OS: WinXP/Pro, Pentium®IV 2.6 GHz (desktop) or Pentium®M 1.6 GHz (laptop), 512Mb RAM. Windows Vista® OS: Vista Business edition, Intel® Core™2 Duo processor, 1 Gb RAM, DirectX 9 capable.
Common: DVD/CD-ROM drive, 80 Gb HD, RS-232 serial port (desktop) or USB port with USB-RS232-serial converter (desktop or laptop), 1024 x 768 monitor (minimum), 1280 x 1024 (preferred), speakers recommended.

Software Extensive analysis, graphing and statistical functions built-in. Unlimited use software licence included, free upgrades (by web download), Windows XP, Pentium IV (2GHz) or higher PC, USB or Serial Port required

40 Cycle Run Time

Typically 45 minutes to 1.5 hours (rotor and protocol dependant)

Supported Volumes

5 µL to 100 µL (rotor and protocol dependant)
Typical reaction volume 20 µL

Instrument Color

Outback Red or Bondi Blue

HRM (High Resolution Melt)

New HRM capability is optional and comprises a dedicated high-performance optical subsystem, additional hardware and specific HRM analysis software (fully integrated). Thermal uniformity ±0.01°C, Resolution ±0.02°C, Range ambient–99°C. HRM data acquisition (read) rate of 20 reads for each 0.02°C increment (=1000 reads/°C)

NOTE: Conventional DNA Melt/Anneal capability and analysis software (including auto-call genotyping capability) is standard on all models.

DNA Concentration Measurement

For direct measurement of nucleic acid concentration using fluorescent dyes (e.g. PicoGreen®, RiboGreen®, etc). Comprises dedicated analysis software (standard on all models)


Rotor-Gene, RotorDisc, HRM and OTV are trademarks of Corbett Life Science and it’s subsidiaries. VIC is a registered trademark and FAM, JOE, ROX and TET are trademarks of Applera Corporation. SYBR, Marina Blue, Alexa Fluor, Texas Red, SYTO, Quant-iT, PicoGreen and RiboGreen are registered trademarks of Invitrogen Corporation, Carlsbad CA. BiosearchBlue is a trademark and CAL Fluor and Quasar are registered trademarks of Biosearch Technologies, Inc. Cy is a registered trademark of GE Healthcare Group Companies. Bothell Blue, Redmond Red and Yakima Yellow are registered trademarks of Epoch Biosciences, Inc. LightCycler Red670, LightCycler Red705 are registered trademarks of Roche Molecular Systems, Inc. LCGreen is a trademark of Idaho Technology Inc. EvaGreen is a trademark of Biotium, Inc. Windows is a registered trademark of Microsoft Corporation. Pentium is a registered trademark of Intel Corporation.

For research use only. Specifications subject to change without notice.
©2007 Corbett Life Science. v2.1

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