Example DNA Extraction Data

The following verification data set demonstrates:
- Purity and yield from a standard whole blood extraction
- Suitability of DNA for downstream mplecular analysis
- No cross-contamination across an entire 96-well plate

Experimental Setup
Whole bovine blood was collected in an EDTA vaccutainer. DNA extractions were made from 200 µL replicates. All samples were processed with a DX Reagent Pack (P/N2220)and an X-tractor Consumable Pack (P/N 2270) according to Whole Blood CorProtocol™ 14101.

A full 96-well plate was processed: 48 positive whole blood and 48 no template control (NTC) samples. As a stringent test for any possibility of well-to-well cross contamination, positive samples (red) and NTCs (blue) were extracted in a checkerboard arrangement as shown below.

Results
Gel electrophoresis of purified extracted DNA from all positive samples showed reproducible yields of high molecular weight DNA migrating alongside a 40 kb DNA size marker in 0.5% agarose (Panel A, opposite). No evidence of degradation (as smearing artefacts) was observed.

Replicate DNA purity and yield of all positive samples was measured spectrophotometrically. Results are summarized in Panel B (opposite).

Real-time amplification analysis was done on all samples targeting the G6PD1 gene. Results show strong reproducible amplification of all positive samples and no evidence of reaction inhibition. No amplification of NTC samples was observed confirming that cross-contamination could not be detected down to the single molecule level (i.e. after 36 amplification cycles for this assay). Results are summarized in Panel C (opposite).

Note: the results shown are indicative only, actual yields will vary depending on the species and the health of the individual from which blood was sampled.