|
Concentration Analysis
The Rotor-Gene 6000 is fully equipped to do concentration analysis from fluorescence measurements. A typical application is the measurement of DNA concentration using PicoGreen dye (see example).
Concentration analysis comprises a standard run protocol and data analysis module included in the standard Rotor-Gene software. In the analysis, the concentration of unknown samples can be determined from prepared standards used to draw a standard curve.
Example of DNA Concentration Measurement
A DNA standard curve with replicates is shown for concentrations of 1000, 900, 800, 700, 600, 500, 400, 300, 200 and 100 pg/µL. Red data points at origin are negative controls. Curve interpolated using a spline curve fit (Rotor-Gene analysis software). Data was obtained using reagents in the Quant-iT™ PicoGreen® dsDNA Kit (Invitrogen Corp., Carlsbad CA). Standard Rotor-Gene concentration analysis run protocol was used. 10 µL PicoGreen® (diluted1/200 in 1 x TE buffer) was combined with 10 µL of each standard (diluted in 1 x TE buffer). Final volume 20 µL. |
|
|
|
